<- Home <- Arhive <- Vol. 25, Issue 4, December 2017

Rom J Leg Med25(4)325-330(2017)
© Romanian Society of Legal Medicine

The variability of C9 expression in ischemic-necrotic lesions of human myocardium

I. Rentea, V. Purcarea, L. Lacramioara, A. Tita, L. E. Ivan, O. Neagu, M. Bosa, Z. Ceausu, M. Ceausu,

Abstract: Sudden cardiac death (SCD) remains one of the major causes of unexpected death. Acute myocardial infarction (MI) before the first 12 hours is always difficult for forensic pathologists. The morphologic changes associated with MI are not often able to estimate accurately when the actual infarct occurred. Our study utilizes the importance of the C9 immunohistochemical biomarker in an attempt to identify this early phase of MI. Different cases with various acute ischemic myocardial lesions, early myocardial infarction and old scarring infarction were investigated in order to establish the certain diagnose of myocardial infarction. The study cases were divided in three groups. Group I with chronic cardiac changes or pathologies, group II with myocardial necrosis and group III for control, with two cases with violent death. For the first group, the immunohistochemical (IHC) reaction was negative in the myocardial fibers as well as rest of the sample, also proving a positive immunohistochemical marking in the vascular region, which validated our results. In the second group, the C9 staining was stronger in the cases with necrotic myocardial fibers, from early stages of the myocardial infarction (24-48 hours and also for 48-72 hours), to lesions 7-10 days old. In the control group (group III) the staining in myocytes was negative. The immunohistochemical expression of C9 has an important diagnosis role for recent myocardial infarction and it could become a standard in forensic laboratory analysis. This method is very useful in forensic practice and can easily determine the diagnostic for recent myocardial infarction.
Keywords: C9 staining, immunohistochemistry, myocardial infarction, hypoxic – ischemic lesions.

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